Mi Hye Song

Courses:

BIO 3232: Biochemistry I
BIO 4240: Mechanisms of Development
BIO 4255: Advanced Methods of Molecular Genetics
BIO 5240: Mechanisms of Development
BIO 5255: Advanced Methods of Molecular Genetics

Research:

Centrosome regulation: Centrosomes are the primary microtubule-organizing centers (MTOCs) in animal cells, which play an instrumental role in cell polarity, intracellular transport, cell motility, and bipolar spindle assembly. While not absolutely required for mitosis, centrosomes normally play a key role in establishing bipolar spindles. Thus, precise regulation of centrosome assembly is crucial for the correct segregation of chromosomes as cells divide. Genomic instability, a feature of many cancers, is often associated with aberrant centrosomes. Recent studies demonstrated that extra centrosomes alone suffice to promote genomic instability via erroneous attachment of chromosome to microtubules, which is mediated by multipolar intermediates. An understanding of the molecular and genetic mechanisms of centrosome assembly should aid our knowledge of human diseases in which this process goes awry. Despite dramatic recent progress, we are still far from a full comprehension of centrosome biogenesis and its link to the cell cycle. Our laboratory is applying a combination of biochemistry, cell biology, genetics, proteomics and quantitative imaging to the study of centrosome biology in the nematode Caenorhabditis elegans model.

Check out this video about Dr. Song's research on YouTube.

Selected Publications:

Mi Hye Song NCBI My Bibliography

Medley* JC, Yim* N, DiPanni* J, Sebou* B, Shaffou* B, Cramer* E, Wu C, Kabara* M, Song MH. (2023). Site-Specific Phosphorylation of ZYG-1 Regulates ZYG-1 Stability and Centrosome Number. iScience, Volume 26, Issue 12, 108410, DOI: 10.1016/j.isci.2023.108410.

Medley* JC, Yim* N, DiPanni* J, Sebou* B, Shaffou* B, Cramer E, Wu C, Kabara* M, Song MH. (2023). Site-Specific Phosphorylation of ZYG-1 Regulates ZYG-1 Stability and Centrosome Number. bioRxiv. 2023 May 30:2023.05.07.539463. DOI: 10.1101/2023.05.07.539463. Preprint. PMID: 37333374.

Medley*, JC and Song, MH (2023). kin-3 genetically suppresses sur-6 in centrosome assembly during Caenorhabditis elegans embryogenesis. microPublication Biology. DOI:10.17912/micropub.biology.000791.

Yim*^#, N., Medley*^# J.C. and M.H. Song. (2022) The C. elegans Casein Kinase II is associated with meiotic DNA in fertilized oocytes. MicroPubl Biol. Jun 6;2022:10.17912/micropub.biology.000583. DOI: 10.17912/micropub.biology.000583 PMCID: PMC9171554. #contributed equally

Medley* J.C., J.R. DiPanni*, L. Schira*, B.M. Shaffou*, B.M. Sebou* and M.H. Song. (2021). APC/CFZR-1 Regulates Centrosomal ZYG-1 to Limit Centrosome Number. J Cell Sci. Jul 15;134(14):jcs253088. DOI: 10.1242/jcs.253088. PMCID: PMC8349554.

Medley*, J.C., DeMeyer*, L.E., Kabara*, M.M. and M.H. Song. (2017). APC/CFZR-1 controls SAS-5 levels to regulate centrosome duplication in Caenorhabditis elegans. G3: Genes, Genomes, Genetics 7 (12): 3937-3946. DOI: 10.1534/g3.117.300260.

DeMeyer*, L., and M.H. Song. (2017). Localization of tbg-1 mRNAs and GFP::TBG-1 protein in early C. elegans embryos. microPublication Biology. DOI: 10.17912/W2CW8H.

Song, M.H, J. C.Medley* and J.Y. Kuwada. (2017). The Zebrafish curly fry mutation is required for proper centrosome and mitotic spindle assembly. Zebrafish 14(4): 311-321. DOI: 10.1089/zeb.2017.1427.

Medley*, J.C., M.M. Kabara*, M.C. Stubenvoll*, L.E. DeMeyer* and M.H. Song. (2017). Casein Kinase II is required for proper cell division and acts as a negative regulator of centrosome duplication in C. elegans embryos. Biology Open 6: 17-28. DOI: 10.1242/bio.022418. Selected for the Biology Open cover photo.

Stubenvoll*, M.D., J.C. Medley*, M. Irwin* and M.H. Song. (2016). ATX-2, the C. elegans ortholog of human Ataxin-2, regulates centrosome size and microtubule dynamics. PLoS Genetics 12(9):e1006370. DOI: 10.1371/journal.pgen.1006370.

Song, M.H.*, Y. Liu, D.E. Anderson and K.F. O’Connell. (2011) Protein Phosphatase 2ASUR-6/B55 Regulates Centriole Duplication by Controlling the Levels of Centriole Assembly Factors ZYG-1 and SAS-5 in C.elegans. Developmental Cell 20(4):563-571. DOI: 10.1016/j.devcel.2011.03.007. PMCID: PMC3079880 *Co-Corresponding Author.

Peters, N., D. Perez, M.H. Song, Y. Liu, T. Müller-Reichert, C. Caron, K. Kemphues and K.F. O'Connell. (2010) Control of Mitotic and Meiotic Centriole Duplication by the Plk4-Related Kinase ZYG-1. J Cell Sci. 123:795-805. doi: DOI: 10.1242/jcs.050682. PMCID: PMC2823580.

Song, M.H., N.B. Miliaras, N. Peel and K.F. O’Connell. (2008) Centrioles: Some Self-Assembly Required. Current Opinion in Cell Biology 20(6):688-693. DOI: 10.1016/j.ceb.2008.09.001.

Song, M.H., L. Aravind, T. Müller-Reichert and K.F. O'Connell. (2008) The Conserved Protein SZY-20 Opposes the Plk4-Related Kinase ZYG-1 to Limit Centrosome Size. Developmental Cell 15(6):901-912. DOI: 10.1016/j.devcel.2008.09.018. PMCID: PMC2829447. **First and Co-Corresponding Author

Kemp, C., M.H. Song, M.K. Addepalli, G. Hunter and K. O’Connell. (2007) Suppressors of zyg-1 define regulators of centrosome duplication and nuclear association in C. elegans. Genetics 176(1):95-113. DOI: 10.1534/genetics.107.071803. (Selected for Issue Highlights). **Co-First Author  

Images selected for MRC London Institute of Medical Science's Biomedical Picture of the Day on January 10, 2017.

*OU student