Department of Biological Sciences

Dodge Hall Rm 375
118 Library Dr
Rochester, MI 48309-4479
(location map)
(248) 370-3550
fax: (248) 370-4225


Anne Hitt
Associate Professor, Ph.D.
301 MSC
(248) 370-4495
Lab location: 331 DH, 332 DH, 333 DH
Lab phone: (248) 370-2896 


  • BIO 111  Biology I
  • BIO 309  Biology of the Cell
  • BIO 325  Biochemistry I
  • BIO 326  Biochemistry I Laboratory
  • BIO 673  Biochemistry of Metabolism and Disease

Research: Cytoskeleton-Membrane Interactions; Membrane Protein Structure and Function

Work in Anne Hitt's lab is targeted towards identification and characterization of proteins that connect the plasma membrane to the underlying cytoskeleton. Such interactions control many fundamental cellular processes, e.g. cell-cell adhesion and signal transduction. Her lab is focusing on the transmembrane protein ponticulin, the major high-affinity link between the actin-based cytoskeleton and the plasma membrane in Dictyostelium. She has cloned the gene for ponticulin from Dictyostelium and subsequently generated a cell line lacking ponticulin by homologous recombination. Currently the structure and biosynthesis of ponticulin are being investigated by site-directed mutagenesis.

Selected Publications:

Hitt, A. L., Iijima-Shimizu, M., DuBay, M.J. Antonette, L.L., Urushihara, H., Wilkerson, C.G. (2003) Identification of a Second Member of the Ponticulin Gene Family and Its Differential Expression Pattern. Biochemica et Biophysica Acta - Gene Structure and Function 1628: 70-87.

Hitt, A. L., Laing, S. D., Olson, S. (2002) Development of a Fluorescent F-actin Blot Overlay Assay for Detection of F-Actin Binding Proteins. Analytical Biochemistry 310:67-71.

Luna, E. J., Hitt, A. L., Shutt, D., Wessels, D., Soll, D., Jay, P., Hug. C., Elson, E. L., Vesley, A., Downey. G. P., Wang, M., Block, S. M., Sigurdson, W., and Sachs, F. (1998) Role of Ponticulin in Pseudopod Dynamics, Cell-Cell Adhesion, and Mechanical Stability of an Ameboid Membrane Skeleton. The Biological Bulletin 194, 345-347.

Luna, E.J., Pestonjamasp, K.N. ,Cheney, R.E., Strassel, C.P., Lu, T.H., Chia, C.P., Hitt, A.L., Fechheimer, M. , Furthmayr, H. , and Mooseker, M.S. (1997) Actin-Binding Membrane Proteins Identified by F-Actin Blot Overlays. Society of General Physiologists Series 52, 3-18.

Shutt, D. C., Wessels, D., Wagenknecht, K., Chandresekhar, A., Hitt, A. L., Luna, E. J., and Soll, D. R. (1995) Ponticulin plays a role in the positional stabilization of pseudopods. Journal of Cell Biology. 131:1495-1506.

Hitt,A.L., Hartwig,J.H., and Luna,E.J. (1994) Ponticulin is the major high-affinity link between the plasma membrane and the cortical actin network in Dictyostelium. Journal of Cell Biology. 126:1433-1444.

Hitt, A. L., Lu, T.-H., and Luna, E. J. (1994) Ponticulin is an atypical membrane protein. Journal of Cell Biology. 126: 1421-1431