Associate Professor, Ph.D.
Lab Location: 331 DHE, 332 DHE, 333 DHE
Degrees: Ph.D. Vanderbilt University, 1988
- BIO 111 Biology I
- BIO 309 Biology of the Cell
- BIO 325 Biochemistry I
- BIO 326 Biochemistry I Laboratory
- BIO 673 Biochemistry of Metabolism and Disease
Research: Cytoskeleton-Membrane Interactions; Membrane Protein Structure and Function
Work in Anne Hitt's lab is targeted towards identification and characterization of proteins that connect the plasma membrane to the underlying cytoskeleton. Such interactions control many fundamental cellular processes, e.g. cell-cell adhesion and signal transduction. Her lab is focusing on the transmembrane protein ponticulin, the major high-affinity link between the actin-based cytoskeleton and the plasma membrane in Dictyostelium. She has cloned the gene for ponticulin from Dictyostelium and subsequently generated a cell line lacking ponticulin by homologous recombination. Currently the structure and biosynthesis of ponticulin are being investigated by site-directed mutagenesis.
Hitt, A. L., Iijima-Shimizu, M., DuBay, M.J. Antonette, L.L., Urushihara, H., Wilkerson, C.G. (2003) Identification of a Second Member of the Ponticulin Gene Family and Its Differential Expression Pattern. Biochemica et Biophysica Acta - Gene Structure and Function 1628: 70-87.
Hitt, A. L., Laing, S. D., Olson, S. (2002) Development of a Fluorescent F-actin Blot Overlay Assay for Detection of F-Actin Binding Proteins. Analytical Biochemistry 310:67-71.
Luna, E. J., Hitt, A. L., Shutt, D., Wessels, D., Soll, D., Jay, P., Hug. C., Elson, E. L., Vesley, A., Downey. G. P., Wang, M., Block, S. M., Sigurdson, W., and Sachs, F. (1998) Role of Ponticulin in Pseudopod Dynamics, Cell-Cell Adhesion, and Mechanical Stability of an Ameboid Membrane Skeleton. The Biological Bulletin 194, 345-347.
Luna, E.J., Pestonjamasp, K.N. ,Cheney, R.E., Strassel, C.P., Lu, T.H., Chia, C.P., Hitt, A.L., Fechheimer, M. , Furthmayr, H. , and Mooseker, M.S. (1997) Actin-Binding Membrane Proteins Identified by F-Actin Blot Overlays. Society of General Physiologists Series 52, 3-18.
Shutt, D. C., Wessels, D., Wagenknecht, K., Chandresekhar, A., Hitt, A. L., Luna, E. J., and Soll, D. R. (1995) Ponticulin plays a role in the positional stabilization of pseudopods. Journal of Cell Biology. 131:1495-1506.
Hitt,A.L., Hartwig,J.H., and Luna,E.J. (1994) Ponticulin is the major high-affinity link between the plasma membrane and the cortical actin network in Dictyostelium. Journal of Cell Biology. 126:1433-1444.
Hitt, A. L., Lu, T.-H., and Luna, E. J. (1994) Ponticulin is an atypical membrane protein. Journal of Cell Biology. 126: 1421-1431