(Rev:01/16/18) Special Animal Safety Protocol [Enter Title of Procedure involving Chemicals or Biohazards]
Investigator Name: IACUC #: Emergency Phone #: Date:	BRSF Approval: ________________________ Date:
EH&S Approval:_________________________ Lab Safety Manager Date:
Animal Safety Hazards	Hazard: [List Toxic Drug, chemical or Biohazard –Virus] Risk Duration: [ Describe how long hazard exists- excrete toxic chemicals or toxic metabolites of these chemicals, and for how many days]
Basic Precautions:	PPE Required: Disposable gown, face mask, shoe covers, nitrile gloves (if required for allergy control or biosafety SOP). Hands must be washed upon exiting animal room using soap and water or other approved hand rub.
Personal Contamination (see Section 9.1 IBC Application)	[Describe how to disinfect anyone exposed to infectious agent or chemical in use.]
Spill Cleanup (see Section 9.3 IBC Application)	[Describe how to disinfect and cleanup a spill of you chemical or biohazardous material.]
Investigator Responsibility
Notification of Animal Care:	Research staff will inform animal care staff ahead of time when hazardous agents will be used, and arrangements will be made for housing of animals. Fresh cages will be used for the animals at the time of administration. This SASP must be posted in the following animal use rooms: [ list applicable BRSF assigned Rooms ]	Signature of research staff who notifies BRSF: _________________________ Date of Notification: _________________________
Posting and labeling Requirements:	This SASP addresses the precautions designed to protect BRSF employees and researchers when handling infectious agents or hazardous chemicals. Danger Sign must be posted on the door of the room in which the animals will be housed while experiments are in progress. Cages with [ List hazard] will be labeled with a [enter color] colored card with the following information: [Name of agent delivered to animal] [Approximate dose] [Date of administration] [Date of bedding change] Cards and door signs must be removed by the researcher once the hazard is no longer present. Per standard lab practice all containers must be labeled including biohazard or cytotoxic chemical labels.
Describe Procedure where animal is potentially exposed to biohazard or chemical and care while infectious or contaminated.	PPE Required: Disposable gown, face mask, shoe covers, nitrile gloves (if required for allergy control or biosafety). Use information from Section 9 of your IBC application. Describe procedure where the infectious agent is introduced into the animal. Description must include the use of the following items if applicable: Plastic screw top jar for secondary containment of vector, or infectious material from main lab to BRSF Lab. Including directions to open in Biosafety Cabinet or Transfer station if necessary. Restraint to control animal (anesthesia, restraint device, etc.) When to use of Biosafety Cabinet or Transfer Station Biohazard labeled waste containers for (Sharps, Biohazard bag, Waste Bottle with for liquids, Waste Bottle for pipette tips. Two Flask Aspirator with Bleach – if necessary Inoculations must be done in a biosafety cabinet. Needles must not be recapped, but if necessary a one handed scoop and tap method is permitted. Describe disinfectant to decontaminate work surface and equipment. Include contact time (example: wipe 70% Ethanol after 20 minutes) and state frequency for preparing fresh bleach solutions (diluted fresh each use, diluted fresh daily, weekly, ect.). Make sure to describe safe handling of sharps Describe procedures for labeling, all containers. If human tissues or human cell lines are used be sure to state that the OU Exposure Control Plan will be followed for any exposures.
The following Sections Describe BRSF Responsibility
Water Bottle Handling:	[If the water becomes biohazardous due to the animal touching bottle, please describe precautions for decontaminating the water bottle and disposal of the liquids in the bottle. Also, note that the bedding may be contaminated by the water bottle liquids and disposal of bedding may need to address this below]
Cage Change:	The first cage change after each drug administration is to be done by the animal care technicians no sooner than___ days after the administration. After this first cage change there is no need for further special precautions to be taken regarding the animals or the cages as long as the animals have not received any more [agent name] . The bedding is considered contaminated and requires special handling. All Personal Protective Equipment (PPE) gown, Nitrile gloves, face mask, shoe covers and cap must be worn to enter animal room. Transfer the animals to clean cages. Replace the tops on the soiled cages and place in a heavyweight plastic bag. Remove the sign from the door of the room housing the animals and the [insert color] cage cards. Items potentially contaminated with agent will be bagged using [red biohazard bags for Biohazards and Yellow Bags for chemical hazards] prior to removal from the animal room. Twist the ends of full bags, goose-neck, and seal with tape. Label with wide tape or other type of label marked [“Cytoxic: Chemical Name.” or Biohazard]. Hands must be washed upon exiting animal room.
Biohazardous Contaminated Soiled Caging, Bedding, and Accessories (BRSF Staff Procedures)	Soiled animal cages, bedding, and accessories contaminated with BSL2 biohazardous agents will be identified as such with red biohazard stickers and placed inside a plastic autoclavable biohazardous waste bag before being removed from the animal housing room. The bag will be tied loosely closed and transported via a wheeled cart to Dirty Cage Processing Room 147 for decontamination using the autoclave. The bag with its contents will be immediately autoclaved using the Unwrapped Load settings (121°C) to ensure proper decontamination and to prevent the cages from melting or discoloring at a higher temperature setting. Do not overfill autoclave bags. Leave space between items to allow for steam circulation. Air pockets or insufficient steam supply will prevent adequate steam saturation. Biohazard bags should be partially open to ensure adequate steam contact. Proper packaging of wastes ensures that steam penetrates. Steam penetration of the load is essential for effective decontamination. Remove the load and let the contents of the load cool for 15 minutes before handling. All caging and their contents are now decontaminated and can be removed from the bag and handled and processed as normal soiled caging is processed. Contaminated bedding must be emptied from cages using Bio-bubble or biosafety cabinet. Disposable gown, face mask, shoe covers, nitrile gloves, and respirator is required for all operators of the Bio-bubble.
Decontamination and Spill Protocol	Empty cages will be sanitized according to current BRSF protocol. Equipment and work surfaces must be washed with soap and water before routine cleaning with [Example:10% freshly prepared bleach] or appropriate disinfectant. All equipment must be decontaminated prior to removal from the room housing the infected animals. Spills will be cleaned up by soaking up liquids with paper towels. Towels contaminated with [agents] will be disposed as biohazardous waste unless directed otherwise by EH&S. materials. Wash affected areas with soap and water before cleaning with 10% freshly prepared bleach or appropriate disinfectant.
Biohazardous Waste (Solid) and Disposal	PPE Required for this Procedure: Disposable gown, face mask, shoe covers, nitrile gloves, and respirator (if required for allergy control or biosafety SOP). All solid BSL2 biohazardous contaminated waste is to only be disposed of in red biohazard labeled containers lined with an autoclavable biohazardous plastic waste bag. Wearing gloves, loosely tie closed the autoclavable biohazardous plastic waste bag lining the red biohazard labeled container. Remove the closed bag from the container and transport to Dirty Cage Processing Room 147 for decontamination using the autoclave. The bag with its contents will be immediately autoclaved using the Unwrapped Load settings (121°C) to ensure proper decontamination of its contents. Remove the load and let the contents of the load cool for 15 minutes before handling. The autoclaved waste (autoclaved bag and its contents) is then placed in a regular black plastic waste bag affixed with a sticker that reads, “Decontaminated Medical Waste”. The decontaminated waste can now be disposed of in the regular trash.
Hazardous Chemical Agents – Chemotherapeutic Agents	PPE Required for this Procedure: Disposable gown, face mask, shoe covers, nitrile gloves (double gloving), safety glasses, and respirator (if required for allergy control or biosafety SOP). All Personal Protective Equipment (PPE): gown, Nitrile gloves (double gloving), face mask, shoe covers and cap must be worn when handling chemotherapeutic contaminated/soiled cages. All chemotherapeutic contaminated/soiled cages will be wiped off with Clidox before removing them from the Transfer Station or Biological Safety Cabinet (BSC), and identified by being labeled with a yellow card tab indicating that animals have been treated with a chemotherapeutic agent, before being transported to Dirty Cage Processing Room 147 for decontamination. Contaminated bedding must be emptied from cages using the bioBubble®. All above PPE is required, in addition to the wearing of N95 masks. Soiled bedding potentially contaminated with agent will be bagged using yellow chemotherapy waste bags prior to removal from the animal facility. Twist the ends of the bags, goose-neck, and seal with wide tape. Dispose of as medical waste, DO NOT AUTOCLAVE chemotherapy waste. Remove all potentially contaminated PPE and place in the chemotherapy waste bag. Hands must be washed upon completion of work and before exiting Dirty Cage Processing. Empty soiled/contaminated cages and accessories will be sprayed with Clidox or 10% bleach and left to sit for a minimum of 10 minutes to decontaminate before processing through the Tunnel Washer or Rack Washer. Employees will wash hands before donning and after removing gloves. An MSDS for the toxic chemical shall be kept in the facility where the animal(s) are housed. All waste containers must always be kept closed (unless adding) to prevent aerosolization and to conform to State and Federal Regulations. Never block access to safety equipment such as safety showers, eyewashes or emergency stop buttons with equipment, carts or racks.