Biochemical Analysis Facilities  

• Miscellaneous
  
  • Flow cytometer system Becton Dickinson FACScan model 210 SEB
• 2-D Electrophoresis
  
  • Quantitative 2-dimensional electrophoresis Bio Rad PROTEAN II xi; Power Supply with CFC-free refrigerating circulator 210 SEB
  • Scanner w/image acquisition and 2-D analysis software w/BioImage software Sun SPARC station: Sun Microsystems 304 DHE
  • Scanner w/image acquisition and 2-D analysis software w/BioImage software Power Mac imaging system 226 SEB
• Microplate Readers
  
  • Fluorescence and luminescence plate reader For Perkin-Elmer spectrofluorometer in 224 SEB 224 SEB
  • Kinetic plate reader Coupled to a Zenith PC with software for various ELISA and other assays 224 SEB
  • Microplate chemiluminometer & spectrofluorometer SPECTRAmax GEMINI. Dual scanning; variable excitation and emission, 250 to 850; fluorescence; luminescence; time-resolved fluorescence. Uses SOFTmax PRO software version 2.6.1. 224 SEB
• Spectrometers
  • Diode array UV/visible spectrophotometer Milton-Roy 3000 with thermal compartment,microprocessor-controlled kinetic functions, MDL 2700 Turbochrom software package and Milton Roy graphics plotter 226 SEB
  • ESR spectrometer New magnet/power supply for existing unit 165 HHS
  • FTIR spectrometer Analect model RFX-30A with a 16 bit 80286 based data system focused beamsampling, system, pyroelectric detector, and diffuse reflectance attachment 335 HHS
  • Gas chromatograph/mass spectrometer Shimadzu model QP-1000 with EI/CI operational externally- switchable source and differential pumping system, sample probe inlet system with heatable sample probe and liquid sample probe. Also with LSS-20 decomissioned library search system with 20 mb Winchester Disk Drive and National Bureau of Standards/Environmental Protection Agency/National Institutes of Health Mass Spectral Data Base. Recent enhancements include PBM Mass Spectrometry Library Search System; Winchester hard drive, and new jet separator operational 1989-1995, decommissioned Fall 1997
  • Spectrofluorometer Perkin-Elmer model LS-5B with stirred cell and microplate reader attachments and software for various applications including free calcium measurements using fura2 and related chelators 224 SEB
  • UV/visible spectrophotometer CIBA-Corning Gilford Response Model with 6 sample thermal compartment and microprocessor- controlled kinetic functions 304A DHE
  • UV/visible spectrophotometer Shimadzu scanning, double beam spectrophotometer with built-in 9.5" LCD and programming features for photometric, kinetics, quantitive, DNA ratios, protein quantitation, spectrum scanning, and data processing 304A DHE
  • UV/visible spectrophotometer Shimadzu model 160U 222 SEB

Biochemical Separations

• Miscellaneous
  
  • Gas chromatograph Hewlett Packard model 5890A 238 SEB
  • HPLC Perkin Elmer Diode-Array LC system equipped with an Omega-2 analytical work station 238 SEB
  • HPLC Perkin Elmer Diode-Array LC system equipped with an isotope flow detector 218 SEB
  • Isoelectric focusing Rotofor purification system with power supply, Bio Rad 210 SEB
  • Rotory evaporator Buchi 202 SEB
• Centrifuges
  
  
  • Centrifuge IEC model PR-7000M, with 6 X 1000 ml Centrac rotor 310 SEB
  • Preparative ultracentrifuge Beckman model L7-65 with type SW 28, 50.1, and 70Ti rotors and VTI 65.1 vertical tube rotor 321 DHE
  • Refrigerated superspeed centrifuge DuPont-Sorvall RC-5B with SS-34 and GSA rotors 321 DHE
  • Refrigerated table top centrifuge Sorvall, with adaptors 308 SEB
  • Ultramicrocentrifuge Beckman Airfug, with refrigeration accessories 202 SEB
• Lyophilization & Concentration
  
  
  • Freeze dryer CT 100 condensing trap with drain, AL90 vacuum pump 308 SEB
  • Maxima vacuum pump 208 SEB
  • Speed vac system Savant Instruments model SS1 208 SEB

Biomedical Electrochemistry Lab

• Bioelectrochemical analyzer 214 SEB
• Blood flowmeter Single channel, ultrasonic transir time, Transonic System 214 SEB
• Incubator Temperature-regulated, Fisher 216 SEB
• Integrator Perkin Elmer model 1022S, single channel 218 SEB
• Laser Doppler flowmeter Transonic Systems model BLF 321 214 SEB
• Microscope Olympus IMT-2 and accessories 216 SEB
• Regulator Alltech, high purity with multipurge 214 SEB
• Syringe pump I/V Programmable, Stoelting 214 SEB
• Ultramicromanipulator Stoelting 214 SEB
• Ventilator Volume controlled, Harvard 214 SEB
• Videomicroscope Olympus & Leco 216 SEB

Cell and Molecular Biology Facilities

• Miscellaneous
  
  • DNA synthesizer MilliGen Cyclone Plus 308 SEB
• Microbial Culture & Low Temperature Freezers
  
  
  • Incubator/shaker Queue model 4730 372 DHE
  • Ultralow temperature (-85C) freezer 17.2 cft upright, Harris model SLT-17V-85 304 DHE
  • Ultralow temperature freezer Harris 226 SEB
• Microscopy
  
  • Confocal microscopy Bio Rad Imaging System 321C DHE
  • Digital camera system Color ??? DHE
  • Fluorescence microscope Zeiss inverted phase contrast 331 SEB
  • Inverted microscopes (2) Nikon TMS 214 SEB & 331 SEB
  • LC PolScope system Microscope application optics package with LC digitial controller and RS-232 interface 168 HH
  • Life support and monitoring system for microsurgery Electrosurgery unit-loop T-pump, pressure model of transducer, standard transducer signal conditioner, L-52 inspection and work station stereoscope, L-57 conversion lens,rectangular base, post stand magnetic base, 3-DIM mechanical joystick manipulator, post mount macromanipulator (left hand), connecting rod, T-pad, lamp, optional L-65 right-lighting system, portable ultraviolet light, Lab PC-2100AI NI-DAQ for Windows, Iomega zip drive (external) 214 SEB
• Tissue Culture Facilities
  
  
  • CO2 incubator Infrared water jacketed Nuaire 210 SEB
  • Cryogenic freezers Standard model & Locator-8: (1800 ampule capacity) 224 SEB
  • Incubator Queue microprocessor controlled model 2720 dual chamber CO2, with CO2 regulators 204 SEB
  • Laminar flow hoods (3) Nuaire with UV light base stand and Magnahelic gauges 210 HHS & 304 DHE
  • Magnetic cell separator 210 SEB

Radioisotope Facilities

• LS 6500 value scintillation system 96 ??? DHE
• Microplate counter Packard Instruments TopCount Microplate (joint with NIH) 224 SEB
• Nuclear counting equipment LKB model 1214-805 excel flexivial liquid scintillation counter with steroid receptor assay program package and DPM program package; LKB Model 1272 gamma counter with PC turbocomputer 308 DHE
• Radio flow detector Perkin-Elmer radio flow detector for high-pressure liquid chromatography (HPLC) 218 SEB
• Radioisotopic imaging system AMBIS Radioanalytic Imaging System 304 DHE